MC-TB 2010 Vol 2, e2
The Effect of Illuminance on Light Induced Reduction of Nitro Blue Tetrazolium
Corresponding author: Ji-Yuan Liang [ liang121@mail.mcu.edu.tw ]
Received 7 October 2010/Revised 12 December 2010/Accepted 23 December 2010/Online published 26 December 2010
Jian-Wei Juen, Hong-Lin Jian, Ji-Yuan Liang*
Department of Biotechnology, School of Health Technology, Ming-Chuan University (Taoyuan, Taiwan, R.O.C.)
Abstract
Superoxide radicals generated from light-exited riboflavin were used to study the effect of illuminance on the light reaction of nitro blue tetrazolium (NBT). The pH value of buffer, illuminance and the concentrations of NBT were found the major factors correlated with the absorbance of the NBT solution after light induced reduction. At a fixed illuminance, the increasing rate of absorbance of NBT reduction is increased with the NBT concentration. At pH 7.8 and higher NBT concentrations, the increasing rates of absorbance of NBT reduction were raised with the enhanced illuminations. However, at lower concentrations of NBT, the available NBT for reaction was limited, though the illuminance had been enhanced. The increasing rates of absorbance of NBT reduction were not increased with the illuminance.. At a same strength of illuminance, the increasing rates of absorbance of NBT reduction at pH 7.8 were higher than those at pH 7.0.
Keyword: riboflavin, NBT, Illuminance
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